How and when to use testicular sperm – Mr Jonathan Ramsay, Imperial College Healthcare NHS Trust, UK

This talk will cover:
• Which non-azoospermic men might benefit from surgical sperm retrieval.
• How to prepare the cases.
• How to achieve best results.
• How to avoid disappointing results.

Mr Jonathan Ramsay is a consultant urologist in West London and an HFEA licence holder at the Andrology Department at Hammersmith Hospital. He has specialised exclusively in the treatment of male fertility for the past 15 years, and more recently has developed an interest in ‘unexplained infertility’.

Since his first publication (with James Nicopoulos) about the relevance of DNA fragmentation, he has recently, with colleagues at Imperial, published widely on the relationships between fragmentation, oxidative stress and genital tract infection. These interests have led to further scrutiny of men with unexplained and secondary infertility who might benefit from surgical sperm retrieval.

Why to use testicular sperm – ART outcomes – Dr James Nicopoullous, The Lister Fertility Clinic, UK

Are microfluidics techniques a better way to choose sperm for ART? – Professor David Smith, University of Birmingham, UK 

This talk aims to:
• Provide an overview of the range of microfluidic techniques available and the state-of-the art in their application to human sperm.
• Discuss cell behaviours in microfluidic environments, in particular boundary and viscosity effects.
• Describe how cell-level events affect population behaviours, and relevance of these effects to cell sorting and preparation for ART.
• Explore future avenues to advance microfluidic preparation of human sperm.

Professor David Smith is a mathematician and theoretical fluid dynamicist by background, having a BA in mathematical sciences from the University of Oxford and a PhD in applied mathematics from the University of Birmingham. Following his PhD he began working with Jackson Kirkman-Brown’s group at the Centre for Human Reproductive Sciences at Birmingham Women’s Hospital, supported by a Medical Research Council training fellowship, developing methods for imaging and analysis of human sperm motility and consideration of fluid dynamical and rheological effects. As a Birmingham Science City Fellow, he subsequently collaborated with Petr Denissenko and Vasily Kantsler (Warwick) on soft lithography-engineered microchannels and their application to human sperm. Now a Professor of Applied Mathematics, he leads an EPSRC-funded team working on a range of biomedical systems, with a strong focus on reproduction and fertility, working closely with Centre for Human Reproductive Sciences.

Female ageing affects the DNA repair capacity of oocytes to amend sperm DNA damage in IVF – potential clinical implications and future directions – Dr Fabrizzio Horta, Monash University, Australia 

This talk aims to:
• Discuss the current evidence of oocyte DNA repair capacity of paternal DNA damage.
• Review in detail the last findings in oocyte DNA repair capacity of sperm DNA damage.
• Discuss potential clinical implications and future directions.

Dr Fabrizzio Horta is a medical reproductive scientist who started his career as a research andrologist. He has been involved in IVF mainly as an andrologist and actively working in the field of IVF as a clinical embryologist. He has undertaken studies in Medical Science, Medicine, Master of Clinical Embryology and PhD studies at Monash University in Melbourne, Australia, fellowships in molecular and clinical andrology as well as training in biomedical innovation and entrepreneurship. He currently works lecturing and developing research at the department of Obstetrics and Gynaecology, School of Clinical Science, Monash University, with graduate students under his supervision as well as developing clinical research at Monash IVF as Clinical Research Embryologist. His current research focus is in male infertility, idiopathic infertility, biomarkers and non-invasive biomarkers of oocyte DNA repair, sperm DNA damage, sperm selection, IVF culture media and technology innovation in IVF.